This practical shows the different ways to search for sequences with keywords in the public databases.

a) Search within UniProtKB. Connect to this URL http://www.uniprot.org

​Find all proteins in Swiss-ProtKB that are less than 4 amino acids
​Do you think the LuxE gene is correct? Why?
​How would you identify the whole sequence?


Try to download the complete proteome of Mycoplasma leachii in FASTA format

How many sequences do you get?

Try to download the reference proteome of Escherichia coli in FASTA format

How many sequences do you get?

Convert these Wormbase IDs into UniProtKB identifiers

WBGene00022613
WBGene00013082
WBGene00014331
WBGene00018433
WBGene00017326
WBGene00196037
WBGene00000690
WBGene00000889
WBGene00021387
WBGene00173418

What happens? Do you get a 1:1 correspondance? Why? 

Last modified: Friday, 22 August 2014, 9:27 AM