NGS - Single-cell RNA-Seq Analysis - November 2019
Section outline
-
NGS - Single-cell RNA-Seq Analysis
Bern, 28-29 November 2019
In room Nr. 304, 3 OG Ost, Universität Bern, Hochschulstrasse 4.
This page is addressed to registered participants. To access the course description and the application form, please click here.
For any assistance, please contact training@sib.swiss.
-
Schedule
First day
Introduction to scRNAseq:
- Technologies
- Experimental design
Quality control
- Dropouts - Doublets
- Ribosomal / mitcochondrial RNAs
Normalization and scalability
- Feature selection
- Log scaling
- confounding factors removal
Second day
Dimentionality reduction and cell type clustering
- PCA
- tSNE
- UMAP
- Clustering methods (Hierarchical, K-means and Graph-based)
Differential expression analysis
- Methods overview
- DE between clusters
- DE between samples (involving data-integration)
Cell type identification
- Methods and applications
Pseudotime analysis
- Methods and applications