Section outline

  • 9:15 Theoretical part (60-90 min):

    intro Prokaryotic epigenetics
    - differences to eukaryotes: more diverse modifications, defined target sequence motifs, RM-systems, ...
    - SMRT sequencing technology, data analysis workflow,
    - brief review of recent literature: applications of SMRT sequencing & findings on biology

    10:30 Coffee break

    11:00 practical session (RStudio, web server)

    - What is the positional resolution of SMRT sequencing, determination of modified bases within specific target motif sequences?
    - How sensitive is SMRT sequencing, compute the fractions of motif instances with modifications detectable by SMRT in bacterial genomes?
    - Are the detected modifications associated with positions of specific genomic features?

    12:30 Lunch Break

    13:30 practical session 2 

    15:00 Coffee break

    15:30 practical session 2