Section outline

  • RNA-seq: From quality control to pathway analysis

    Bern, 15-16 February 2016

    Trainers: Walid Gharib, Geoffrey Fucile, Irene Keller
    Venue: University of Bern
    ECTS: 0.5 (given a passed exam at the end of the course)
    Fee: 100 CHF for academics. Others, please contact us
    Application deadline: 4 February 2016
    Application status: CLOSED

    Overview

    This course will present all the bioinformatics tools required to analyze RNA-seq gene expression data, from the raw data to the biological interpretation. This two-day course will discuss the following topics:
    • Quality control and reads cleanup
    • RNAseq reads mapping to genome & transcriptome
    • Gene reads counting, gene & exons differential expression
    • GO enrichment and pathway analysis

    Learning objectives

    At the end of the course attendees will:

    • Understand advantages and pitfalls for RNA sequencing
    • Be able to design their own experiment
    • Practise the downstream analysis using command line software (QC, mapping, counting, Diff Expression, gene enrichment, pathway analysis)

    Prerequisites

    Knowledge / competencies:

    • Participants should already have a basic knowledge in Next Generation Sequencing (NGS) techniques or already followed the Introduction to NGS course;
    • A basic knowledge of the R statistical software (http://www.r-project.org/) is also required.

    Location

    Nr. 215 / 2. OG West - Universität Bern, Hochschulstrasse 4

    Additional information

    Coordination: Diana Marek - SIB Training and Outreach

    You are welcome to register to the SIB courses mailing-list to be informed of all future courses and workshops, as well as all important deadlines using the form here.

    For more information, please contact training@isb-sib.ch.