For the last few years, research in DNA and RNA has been dominated by short-read sequencing. Although this has revolutionized the way we answer biological questions, it has its limitations. One important limitation is read length. The invention of high-throughput long-read sequencing has enabled us to solve questions on genome assembly, haplotyping, structural variation and alternative splicing. In this course, the participant will get acquainted with the major long-read sequencing technologies (PacBio SMRT and Oxford Nanopore Technology), and get experience with hands-on work involving long-read data.

This course is addressed to people that are working with or will be working with long-read data.

At the end of the course, the participants are expected to:

• Understand the basics behind PacBio SMRT sequencing and Oxford Nanopore Technology sequencing
• Use the command line to perform quality control and read alignment of long-read sequencing data
• Be able to do differential isoform expression analysis or a repeat expansion analysis based on long-read sequencing data