Methylations of the DNA are important modifications that affect the DNA of bacteria and higher eucaryotes. The DNA can be modified on different bases (6mA, 4mC, 5mC) and these modifications have various effects, e.g., on gene expression or protection against foreign DNA. These modifications can be transmitted from generation to generation, they are called epigenetics modifications. Current next generation sequencing technologies allow for the detection of those modifications at the genome level introducing the « methylome ». In addition Histone modifications are also part of the epigenomics modifications that can influence gene expression. These modification can be analyzed by NGS methods. In this workshop we will study the methods used to produce and to analyze this NGS data.