The goal of this one-day workshop is to give some basic theoretical and practical knowledge on protein sequence databases and, in particular, on the optimum use of the UniProt web site:

  • Where do the protein sequences come from?
  • What are the differences between UniProtKB/TrEMBL and UniProtKB/Swiss-Prot?
  • What the manual and automated annotation pipelines ?
  • How to estimate the protein sequence accuracy and the annotation quality?
  • How to retrieve large dataset (i.e. complete proteome) for a particular organism?
  • How to extract biological knowledge from a Blast result?
  • What are the differences between UniProt and the NCBI protein sequence databases?
  • How to retrieve accession numbers or GIs or other record identifiers corresponding to the proteins of interest?