Using web-based tools for Bioinformatics research is very convenient for small amounts of data (e.g. less than 20 sequences). As soon as researchers want to analyse more sequences (from 20 up to thousands sequences or megabytes of data), the task becomes tedious. The course aims at providing the basic knowledge of PERL scripting abilities to analyse large amounts of data by automating recurring tasks or by grouping several tasks into one program. This course is organised for biomedical researchers and no particular programming knowledge is required, however some basic Unix/Linux computing skills would be of great advantage.